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Boster Bio Anti-GCS-beta-2 Antibody (Catalog# A13667). Tested in IHC-P, IF (paraffin), ELISA applications. This antibody reacts with Human, Mouse, Rat.
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Image Search Results
Journal: Frontiers in endocrinology
Article Title: Specific dilation pattern in placental circulation and the NO/sGC role in preeclampsia placental vessels.
doi: 10.3389/fendo.2023.1182636
Figure Lengend Snippet: FIGURE 5 Role of NO/sGC in regulating the baseline tone of placental and non-placental vessels. On the baseline of vessel tone, SNP mediated dilation in several species (A—human: normal placental vessels (CPV, n = 25) and umbilical vessels (CUV, n = 25); B—sheep: placental vessels (SPV, n = 16), umbilical vessels (SUV, n = 11), and fetal sheep mesenteric arteries (SMA, n = 4), C–rat: umbilical vessels and mesenteric arteries) with or without ODQ. Bay 41-2272 induced dilation in CPV (n = 12) and CUV (n = 5) (A). The protein expressions of GUCY1A3, GUCY1B3, cGMP, and PRKG1 in placental vessels and umbilical vessels were shown (D). The real-time recording images were also shown. *P <0.05; ***P <0.001; ****P <0.0001; ###P <0.001; ####P <0.0001.
Article Snippet: The primary
Techniques:
Journal: Frontiers in endocrinology
Article Title: Specific dilation pattern in placental circulation and the NO/sGC role in preeclampsia placental vessels.
doi: 10.3389/fendo.2023.1182636
Figure Lengend Snippet: FIGURE 6 Dilation in placental vessels in normal and preeclamptic pregnancy and related mechanisms. The concentrations of NO in placental vessels and umbilical plasma from both groups were shown (A). The mRNA and protein expressions of eNOS as well as the phosphorylated eNOS are shown (A). On the 5HT-induced vasoconstriction platform, concentration-dependent SNPs, or Bay 41-2272 induced dilation responses in placental vessels from both groups (B). On the baseline of vessel tone, SNP caused dilation in the presence or absence of ODQ (C). Bay 41-2272 decreased the baseline of placental vessel tone (C). The sGC activity and protein expressions of GUCY1A3, GUCY1B3, and PRKG1 were shown (D). *P <0.05; **P <0.01; ***P <0.001; ****P <0.0001; ####P <0.0001.
Article Snippet: The primary
Techniques: Clinical Proteomics, Concentration Assay, Activity Assay
Journal: International journal of molecular sciences
Article Title: Arsenite-Mediated Transcriptional Regulation of Glutathione Synthesis in Mammalian Primary Cortical Astrocytes.
doi: 10.3390/ijms26115375
Figure Lengend Snippet: Figure 3. Effect of arsenite on astrocyte GCL mRNA and protein expression. Primary cortical astrocytes (n = 3 from 3 separate dissections) were treated with 10 µg/mL ActD (+ActD; gray bars) or its vehicle (−ActD; blue bars) for 30 min, after which NaAsO2 was spiked into experimental wells to a final concentration of 15µM. Four hours later, RNA was isolated, and (A) GCLC and (D) GCLM mRNA expression was determined by RT-qPCR. Data are expressed as mean + SEM fold change over untreated cells (−NaAsO2, −ActD; =1). Exact p-values are shown for each pairwise comparison determined by two-way ANOVA followed by Fisher’s uncorrected LSD test for multiple comparisons. Protein expression of (B) GCLC and (E) GCLM was assessed by Western blotting, as described in the Section 4. Exact p-values are shown for each pairwise comparison determined to be significant by a paired t-test. Representative blots for (C) GCLC and (F) GCLM are shown.
Article Snippet: After destaining, membranes were blocked in Odyssey blocking buffer (LI-COR Environmental, Lincoln, NE, USA) for 1 h at room temperature, followed by overnight incubation at 4 ◦C with primary
Techniques: Expressing, Concentration Assay, Isolation, Quantitative RT-PCR, Comparison, Western Blot